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Testing for Leaky Gut


There are two ways to medically test leaky gut and, more importantly, some of its effects.

  1. The standard leaky gut Lab test is called the Lactulose/Mannitol Challenge. You drink a solution containing two substances (non-metabolised sugar molecules lactulose and mannitol) and then collect your urine. Lactulose is large and penetrates poorly through the intestinal barrier so an elevated level of lactulose in your urine is indicative of increased intestinal permeability. Mannitol, on the other hand, is usually well absorbed so a low percentage recovery of mannitol is indicative of malabsorption. A high lactulose/mannitol ratio means that you have increased gut permeability.
  2. Another test, perhaps more valuable, is to measure the effects of leaky gut with a food allergy test, specifically IgE, IgG4 and IgA antibodies. There is scientific certainty that leaky gut will cause food sensitivities. If leaky gut is to be healed, those foods must be identified and removed.


Allergic and other hypersensitivity reactions to foods and aeroallergens are characterized by elevated allergen-specific antibody levels in the affected individual’s blood. Research indicates that these types of reactions are implicated in a number of health problems. There are MANY - several dozen - reactions ranging from an immune based food allergy.